Rapid and Scalable Plant-Based Production of a Potent Plasmin Inhibitor Peptide.
Mark A Jackson, Kuok Yap, Aaron G Poth, Edward K Gilding, Joakim E Swedberg, Simon Poon, Haiou Qu, Thomas Durek, Karen Harris, Marilyn A Anderson, David J Craik
Front Plant Sci | Published : 2019
The backbone cyclic and disulfide bridged sunflower trypsin inhibitor-1 (SFTI-1) peptide is a proven effective scaffold for a range of peptide therapeutics. For production at laboratory scale, solid phase peptide synthesis techniques are widely used, but these synthetic approaches are costly and environmentally taxing at large scale. Here, we developed a plant-based approach for the recombinant production of SFTI-1-based peptide drugs. We show that transient expression in Nicotiana benthamiana allows for rapid peptide production, provided that asparaginyl endopeptidase enzymes with peptide-ligase functionality are co-expressed with the substrate peptide gene. Without co-expression, no target..View full abstract