Examination of the Interaction between a Membrane Active Peptide and Artificial Bilayers by Dual Polarisation Interferometry

Jennifer AE Payne; Tzong-Hsien Lee; Marilyn A Anderson; Marie-Isabel Aguilar

Journal article

Examination of the Interaction between a Membrane Active Peptide and Artificial Bilayers by Dual Polarisation Interferometry

Jennifer AE Payne, Tzong-Hsien Lee, Marilyn A Anderson, Marie-Isabel Aguilar

BIO-PROTOCOL | BIO-PROTOCOL | Published : 2017

DOI: 10.21769/BioProtoc.2087

Abstract

Examining the interaction of peptides with lipid bilayers to determine binding kinetics is often performed using surface plasmon resonance (SPR). Here we describe the technique of dual polarisation interferometry (DPI) that provides not only information on the kinetics of the peptide binding to the bilayer, but also how the peptide affects the lipid order of the bilayer.

University of Melbourne Researchers

Marilyn Anderson Author

Related Projects (1)

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Cytolytic antimicrobial peptides (AMPs) are key components of the innate immune system of many organisms including man. They act by disrupti..

Grants

Awarded by Australian Research Council

Awarded by National Health & Medical Research Council

Funding Acknowledgements

MAA is funded by the Australian Research Council (DP150104386). MIA acknowledges the support of the National Health & Medical Research Council (#1044327) and the Australian Research Council (DP1110101866). This protocol was adapted from the work of Lee et al. (2010) and Payne et al. (2016).