Biochemical and Functional Characterization of GALT8, an Arabidopsis GT31 beta-(1,3)-Galactosyltransferase That Influences Seedling Development
Joan Onate Narciso, Wei Zeng, Kris Ford, Edwin R Lampugnani, John Humphries, Ingvild Austarheim, Allison van de Meene, Antony Bacic, Monika S Doblin
FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2021
Arabinogalactan-proteins (AGPs) are members of the hydroxyproline-rich glycoprotein (HRGP) superfamily, a group of highly diverse proteoglycans that are present in the cell wall, plasma membrane as well as secretions of almost all plants, with important roles in many developmental processes. The role of GALT8 (At1g22015), a Glycosyltransferase-31 (GT31) family member of the Carbohydrate-Active Enzyme database (CAZy), was examined by biochemical characterization and phenotypic analysis of a galt8 mutant line. To characterize its catalytic function, GALT8 was heterologously expressed in tobacco leaves and its enzymatic activity tested. GALT8 was shown to be a β-(1,3)-galactosyltransferase (Gal..View full abstract
Awarded by Australia Research Council (ARC)
Awarded by Overseas Expertise Introduction Project for Discipline Innovation "111" project
WZ, EL, KF, JH, AvdM, MD, and AB acknowledge the support of a grant from the Australia Research Council (ARC) to the ARC Centre of Excellence in Plant Cell Walls [CE1101007]. WZ, MD, and AB acknowledge the support from the Overseas Expertise Introduction Project for Discipline Innovation "111" project (D18008). JON acknowledges the support of the University of Melbourne MRS and MIFRS scholarships. EL and JH acknowledge funding from the University of Melbourne, Faculty of Science.