High-throughput microfluidic compressibility cytometry using multi-tilted-angle surface acoustic wave
Yanqi Wu, Alastair G Stewart, Peter VS Lee
LAB ON A CHIP | ROYAL SOC CHEMISTRY | Published : 2021
Cellular mechanical properties (e.g. compressibility) are important biophysical markers in relation to cellular processes and functionality. Among the methods for cell mechanical measurement, acoustofluidic methods appear to be advantageous due to tunability, biocompatibility and acousto-mechanical nature. However, the previous acoustofluidic methods were limited in throughput and number of measurements. In this study, we developed a high-throughput microfluidic compressibility cytometry approach using multi-tilted-angle surface acoustic wave, which can provide thousands of single-cell compressibility measurements within minutes. The compressibility cytometer was constructed to drag micropar..View full abstract
Awarded by Australian Research Council
We thank Yudi Niu for helping build the simulation model. This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). We acknowledge financial support from the Australian Research Council (IC17010000016) support of the ARC Centre for Personalised Therapeutics Technologies and the work contained in this study.