Journal article

Crumbs and the apical spectrin cytoskeleton regulate r8 cell fate in the Drosophila eye

Jonathan M Pojer, Abdul Jabbar Saiful Hilmi, Shu Kondo, Kieran F Harvey

PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2021

Abstract

The Hippo pathway is an important regulator of organ growth and cell fate. In the R8 photoreceptor cells of the Drosophila melanogaster eye, the Hippo pathway controls the fate choice between one of two subtypes that express either the blue light-sensitive Rhodopsin 5 (Hippo inactive R8 subtype) or the green light-sensitive Rhodopsin 6 (Hippo active R8 subtype). The degree to which the mechanism of Hippo signal transduction and the proteins that mediate it are conserved in organ growth and R8 cell fate choice is currently unclear. Here, we identify Crumbs and the apical spectrin cytoskeleton as regulators of R8 cell fate. By contrast, other proteins that influence Hippo-dependent organ growt..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Research Council


Funding Acknowledgements

K.F.H was supported by the National Health and Medical Research Council of Australia (1078220 and 1194467) (https://www.nhmrc.gov.au/).J.M.P.was supported by an Australian Postgraduate Award (https://scholarships.unimelb.edu.au/awards/graduate-research-scholarships).This research was supported by a grant to K.F.H. from the Australian Research Council (DP180102044) (https://www.arc.gov.au/).The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.We thank members of the Harvey lab for discussions. We thank C. Desplan, R. Johnston, H. Richardson, K. Irvine, the Bloomington Drosophila Stock Center, the Vienna Drosophila RNAi Center, the Kyoto Stock Center, the National Institute of Genetics (Japan), the Australian Drosophila Research Support Facility (www.ozdros.com), and the Developmental Studies Hybridoma Bank for D. melanogaster stocks and antibodies. We acknowledge the Peter Mac Centre for Advanced Histology and Microscopy and support to them from the Peter MacCallum Cancer Foundation and the Australian Cancer Research Foundation.