Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome.
Daniel Fox, Anukriti Mathur, Yansong Xue, Yunqi Liu, Wei Hong Tan, Shouya Feng, Abhimanu Pandey, Chinh Ngo, Jenni A Hayward, Ines I Atmosukarto, Jason D Price, Matthew D Johnson, Nadja Jessberger, Avril AB Robertson, Gaetan Burgio, David C Tscharke, Edward M Fox, Denisse L Leyton, Nadeem O Kaakoush, Erwin Märtlbauer Show all
Nat Commun | Published : 2020
Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of the NLRP3 inflammasome and pyroptosis. NHE is a non-redundant toxin to haemolysin BL (HBL) despite having a similar mechanism of action. Via a putative transmembrane region, subunit C of NHE initiates binding to the plasma membrane, leading to the recruitment of subunit B and subunit A, thus forming a tripartite lytic pore that is permissive to efflux of potassium. NHE mediates killing of cells from multiple lineages and hosts, highlighting a versatile functional re..View full abstract