Conjugated Polyelectrolyte Nanoparticles for Apoptotic Cell Imaging.
Yu Liu, Pan Wu, Jianhua Jiang, Jiatao Wu, Yan Chen, Ying Tan, Chunyan Tan, Yuyang Jiang
ACS Appl Mater Interfaces | Published : 2016
Three anionic conjugated polyelectrolytes (CPEs) with poly(p-phenylene ethynylene thiophene) backbones were designed and synthesized, among which PPET3-CO2Na showed greater molar extinction coefficient with red-shifted bands in both absorption and emission spectra compared to the well-studied PPE-CO2Na polymer. PPET3-CO2Na was thus chosen to construct CPE-based nanoparticles (CPNs) with cationic octaarginine (R8) peptide through electrostatic-interaction-induced self-assembly. Due to plasma membrane permeabilization and mitochondrial outer membrane permeabilization (MOMP) in early apoptotic cells, PPET3/R8 CPNs demonstrated excellent colocalization with MitoTracker Red in apoptotic cells ins..View full abstract