Host cell transcriptome profile during wild-type and attenuated dengue virus infection.
October M Sessions, Ying Tan, Kenneth C Goh, Yujing Liu, Patrick Tan, Steve Rozen, Eng Eong Ooi
PLoS Negl Trop Dis | Published : 2013
Dengue viruses 1-4 (DENV1-4) rely heavily on the host cell machinery to complete their life cycle, while at the same time evade the host response that could restrict their replication efficiency. These requirements may account for much of the broad gene-level changes to the host transcriptome upon DENV infection. However, host gene function is also regulated through transcriptional start site (TSS) selection and post-transcriptional modification to the RNA that give rise to multiple gene isoforms. The roles these processes play in the host response to dengue infection have not been explored. In the present study, we utilized RNA sequencing (RNAseq) to identify novel transcript variations in ..View full abstract