Journal article

The RNA-Binding Proteins SRP14 and HMGB3 Control HIV-1 Tat mRNA Processing and Translation During HIV-1 Latency

Georges Khoury, Michelle Y Lee, Sri H Ramarathinam, James McMahon, Anthony W Purcell, Secondo Sonza, Sharon R Lewin, Damian FJ Purcell

FRONTIERS IN GENETICS | FRONTIERS MEDIA SA | Published : 2021

Abstract

HIV-1 Tat protein is essential for virus production. RNA-binding proteins that facilitate Tat production may be absent or downregulated in resting CD4+ T-cells, the main reservoir of latent HIV in people with HIV (PWH) on antiretroviral therapy (ART). In this study, we examined the role of Tat RNA-binding proteins on the expression of Tat and control of latent and productive infection. Affinity purification coupled with mass spectrometry analysis was used to detect binding partners of MS2-tagged tat mRNA in a T cell-line model of HIV latency. The effect of knockdown and overexpression of the proteins of interest on Tat transactivation and translation was assessed by luciferase-based reporter..

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Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC) program


Awarded by NHMRC Principal Research Fellowship


Funding Acknowledgements

This study was supported by the National Health and Medical Research Council of Australia (NHMRC) program and project grants (#1052979 and #1129320 to DP), NHMRC Principal Research Fellowship (#1137739 to AP) and Australian Centre for HIV and Hepatitis Virology Research (ACH2) (2015-21 to DP).