Journal article
Single cell transcriptome atlas of mouse mammary epithelial cells across development
B Pal, Y Chen, MJG Milevskiy, F Vaillant, L Prokopuk, CA Dawson, BD Capaldo, X Song, F Jackling, P Timpson, GJ Lindeman, GK Smyth, JE Visvader
Breast Cancer Research | BMC | Published : 2021
Abstract
Background: Heterogeneity within the mouse mammary epithelium and potential lineage relationships have been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during mammary ontogeny, we profiled single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, and post-involution, as well as the transcriptomes of micro-dissected terminal end buds (TEBs) and subtending ducts during puberty. Methods: The single cell transcriptomes of 132,599 mammary epithelial cells from 9 different developmental stages were determined on the 10x Genomics Chromium platform, and..
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Awarded by Ian Potter Foundation
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council (NHMRC), grants #1016701, #1054618, #1100807, #1113133; NHMRC IRIISS; Chan Zuckerberg Initiative EOSS; the Victorian State Government through Victoria Cancer Agency Funding and Operational Infrastructure Support; the National Breast Cancer Foundation (IIRS-20-022); the Australian Cancer Research Foundation and the Ian Potter Foundation; M.M. by VCA Fellowship ECRF19011; Y.C. by MRFF Investigator Grant #1176199; G.J.L. and G.K.S. by NHMRC Fellowships #1078730, #1058892; and J.E.V. by NHMRC Fellowships #1037230 and 1102742.