Journal article

Single cell transcriptome atlas of mouse mammary epithelial cells across development

Bhupinder Pal, Yunshun Chen, Michael JG Milevskiy, Francois Vaillant, Lexie Prokopuk, Caleb A Dawson, Bianca D Capaldo, Xiaoyu Song, Felicity Jackling, Paul Timpson, Geoffrey J Lindeman, Gordon K Smyth, Jane E Visvader



BACKGROUND: Heterogeneity within the mouse mammary epithelium and potential lineage relationships have been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during mammary ontogeny, we profiled single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, and post-involution, as well as the transcriptomes of micro-dissected terminal end buds (TEBs) and subtending ducts during puberty. METHODS: The single cell transcriptomes of 132,599 mammary epithelial cells from 9 different developmental stages were determined on the 10x Genomics Chromium platform, and..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by National Breast Cancer Foundation

Awarded by VCA Fellowship

Awarded by MRFF

Awarded by NHMRC

Funding Acknowledgements

This work was supported by the Australian National Health and Medical Research Council (NHMRC), grants #1016701, #1054618, #1100807, #1113133; NHMRC IRIISS; Chan Zuckerberg Initiative EOSS; the Victorian State Government through Victoria Cancer Agency Funding and Operational Infrastructure Support; the National Breast Cancer Foundation (IIRS-20-022); the Australian Cancer Research Foundation and the Ian Potter Foundation; M.M. by VCA Fellowship ECRF19011; Y.C. by MRFF Investigator Grant #1176199; G.J.L. and G.K.S. by NHMRC Fellowships #1078730, #1058892; and J.E.V. by NHMRC Fellowships #1037230 and 1102742.