Journal article
Carbon Monoxide is an Inhibitor of HIF Prolyl Hydroxylase Domain 2
NM Mbenza, N Nasarudin, PG Vadakkedath, K Patel, AZ Ismail, M Hanif, LJ Wright, V Sarojini, CG Hartinger, IKH Leung
Chembiochem | Published : 2021
Abstract
Hypoxia-inducible factor prolyl hydroxylase domain 2 (PHD2) is an important oxygen sensor in animals. By using the CO-releasing molecule-2 (CORM-2) as an in situ CO donor, we demonstrate that CO is an inhibitor of PHD2. This report provides further evidence about the emerging role of CO in oxygen sensing and homeostasis.
Grants
Funding Acknowledgements
We thank the University of Auckland for funding. N.M.M. was supported by grant-in-aid scholarships funded by the School of Chemical Sciences of the University of Auckland. N.N. was supported by a Science Education Teaching Scheme funded by the Majlis Amanah Rakyat Agency (MARA). P.G.V. was supported by a doctoral scholarship funded by the MacDiarmid Institute for Advanced Materials and Nanotechnology. A.Z.I. would like to acknowledge the financial support from the Malaysian Ministry of Higher Education (MOHE) under the Academic Training Scheme SLAB-KPT/2013-2016. M.H. is supported by a Sir Charles Hercus Health Research Fellowship through the Health Research Council of New Zealand. We thank Dr Ryan Dixon (NMR spectroscopy), Martin Middleditch (protein mass spectrometry), Dr Gus Grey (MALDI-TOF mass spectrometry) and Dr Mansa Nair (nondenaturing nano-ESI mass spectrometry) for technical support. There are no conflicts to declare.