Journal article

Definition of the immune evasion-replication interface of rabies virus P protein

Jingyu Zhan, Angela R Harrison, Stephanie Portelli, Thanh Binh Nguyen, Isshu Kojima, Siqiong Zheng, Fei Yan, Tatsunori Masatani, Stephen M Rawlinson, Ashish Sethi, Naoto Ito, David B Ascher, Gregory W Moseley, Paul R Gooley

PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2021

Abstract

Rabies virus phosphoprotein (P protein) is a multifunctional protein that plays key roles in replication as the polymerase cofactor that binds to the complex of viral genomic RNA and the nucleoprotein (N protein), and in evading the innate immune response by binding to STAT transcription factors. These interactions are mediated by the C-terminal domain of P (PCTD). The colocation of these binding sites in the small globular PCTD raises the question of how these interactions underlying replication and immune evasion, central to viral infection, are coordinated and, potentially, coregulated. While direct data on the binding interface of the PCTD for STAT1 is available, the lack of direct struc..

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Grants

Awarded by National Health & Medical Research Council Australia


Awarded by Australian Research Council


Funding Acknowledgements

This research was supported by National Health & Medical Research Council Australia grants (https://www.nhmrc.gov.au/) 1125704 (G. W.M and P.R.G), 1079211, 1003244, 1160838 (G. W.M), 1174405 (D.B.A and T.B.N), Australian Research Council discovery project grant (https://www.arc.gov.au/) DP150102569 (G.W.M), Meigunyah Trust Grimwade Fellowship (G.W.M), Australian Government Research Training Program Scholarship (A.R.H) and Melbourne Research Scholarships (J.Z. and S.P.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.