Journal article
Tissue-specific expression of Cas9 has no impact on whole-body metabolism in four transgenic mouse lines
ST Bond, A Zhuang, C Yang, EAM Gould, T Sikora, Y Liu, Y Fu, KI Watt, Y Tan, H Kiriazis, GI Lancaster, P Gregorevic, DC Henstridge, JR McMullen, PJ Meikle, AC Calkin, BG Drew
Molecular Metabolism | Published : 2021
Abstract
Objective: CRISPR/Cas9 technology has revolutionized gene editing and fast tracked our capacity to manipulate genes of interest for the benefit of both research and therapeutic applications. Whilst many advances have, and continue to be made in this area, perhaps the most utilized technology to date has been the generation of knockout cells, tissues and animals. The advantages of this technology are many fold, however some questions still remain regarding the effects that long term expression of foreign proteins such as Cas9, have on mammalian cell function. Several studies have proposed that chronic overexpression of Cas9, with or without its accompanying guide RNAs, may have deleterious ef..
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Awarded by State Government of Victoria
Funding Acknowledgements
We acknowledge funding support from the Victorian State Government OIS program to Baker Heart & Diabetes Institute. These studies were supported by funding from the Baker Heine Trust through both the Obesity & Lipid and the Bioinformatics Programs, as well as the Bertalli mini-grant scheme at Baker. During the period of this study, some authors were supported by fellowship funding as follows: BGD and ACC, National Heart Foundation of Australia (101789 and 100067, respectively) ; JRMand PG, NHMRC of Australia (APP1078985 and APP1117835, respectively) . We thank members of the MMA, LMCD, Cardiac Hypertrophy, Metabolomics, and Hematopoiesis & Leukocyte Biology laboratories at BHDI for their contributions. We also acknowledge the use of the facilities and technical assistance of the Monash Histology Platform, Department of Anatomy and Developmental Biology, Monash University. Figure 1A was created with Biorender (Biorender.com ) .