Dihydrosphingosine driven enrichment of sphingolipids attenuates TGF beta induced collagen synthesis in cardiac fibroblasts
Ruth R Magaye, Feby Savira, Xin Xiong, Kevin Huynh, Peter J Meikle, Christopher Reid, Bernard L Flynn, David Kaye, Danny Liew, Bing H Wang
IJC HEART & VASCULATURE | ELSEVIER IRELAND LTD | Published : 2021
The sphingolipid de novo synthesis pathway, encompassing the sphingolipids, the enzymes and the cell membrane receptors, are being investigated for their role in diseases and as potential therapeutic targets. The intermediate sphingolipids such as dihydrosphingosine (dhSph) and sphingosine (Sph) have not been investigated due to them being thought of as precursors to other more active lipids such as ceramide (Cer) and sphingosine 1 phosphate (S1P). Here we investigated their effects in terms of collagen synthesis in primary rat neonatal cardiac fibroblasts (NCFs). Our results in NCFs showed that both dhSph and Sph did not induce collagen synthesis, whilst dhSph reduced collagen synthesis ind..View full abstract
Awarded by National Health and Medical Research Council of Australia
This research was supported by National Health and Medical Research Council of Australia (Program Grants 1092642 and Project Grant 1087355). RM and FS are supported by the Monash University International Post Graduate Research Scholarships and Monash Graduate Scholarships.