Journal article

Towards spatio-temporally resolved developmental cardiac gene regulatory networks in zebrafish

JC Hallab, HT Nim, J Stolper, G Chahal, L Waylen, F Bolk, DA Elliott, E Porrello, M Ramialison

Briefings in Functional Genomics | OXFORD UNIV PRESS | Published : 2021

Open access

Abstract

Heart formation in the zebrafish involves a rapid, complex series of morphogenetic events in three-dimensional space that spans cardiac lineage specification through to chamber formation and maturation. This process is tightly orchestrated by a cardiac gene regulatory network (GRN), which ensures the precise spatio-temporal deployment of genes critical for heart formation. Alterations of the timing or spatial localisation of gene expression can have a significant impact in cardiac ontogeny and may lead to heart malformations. Hence, a better understanding of the cellular and molecular basis of congenital heart disease relies on understanding the behaviour of cardiac GRNs with precise spatiot..

View full abstract

Grants

Awarded by Royal Children's Hospital Foundation


Funding Acknowledgements

We thank the members of the Ramialison Laboratory for their support. This project is funded by the Australian Research Council Discovery Project DP190102771 to M.R. and GS is funded by a Rotary Global Grant, Rotary Foundation International [GG1637399]. Further support was received from the National Health and Medical Research Council of Australia (D.A.E., E.R.P.), Australian Research Council (E.R.P., M.R.), Heart Foundation of Australia (D.A.E.), The Stafford Fox Medical Research Foundation (D.A.E., E.R.P., M.R.) and the Royal Children's Hospital Foundation (D.A.E., E.R.P.). Murdoch Childrens Research Institute supported by Victorian Government's Operational Infrastructure Support Program and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme (NHMRC IRIISS). The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government.