Practical High-Throughput Method to Screen Compounds for Anthelmintic Activity against Caenorhabditis elegans
Aya C Taki, Joseph J Byrne, Peter R Boag, Abdul Jabbar, Robin B Gasser
MOLECULES | MDPI | Published : 2021
In the present study, we established a practical and cost-effective high throughput screening assay, which relies on the measurement of the motility of Caenorhabditis elegans by infrared light-interference. Using this assay, we screened 14,400 small molecules from the "HitFinder" library (Maybridge), achieving a hit rate of 0.3%. We identified small molecules that reproducibly inhibited the motility of C. elegans (young adults) and assessed dose relationships for a subset of compounds. Future work will critically evaluate the potential of some of these hits as candidates for subsequent optimisation or repurposing as nematocides or nematostats. This high throughput screening assay has the adv..View full abstract
The present study was supported by the Australian Research Council (ARC), Phylumtech (Argentina) and Yourgene Health p/l (Singapore).