Journal article
Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node
Isaac M Barber-Axthelm, Hannah G Kelly, Robyn Esterbauer, Kathleen M Wragg, Anne M Gibbon, Wen Shi Lee, Adam K Wheatley, Stephen J Kent, Hyon-Xhi Tan, Jennifer A Juno
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2021
Abstract
Characterization of germinal center B and T cell responses yields critical insights into vaccine immunogenicity. Nonhuman primates are a key preclinical animal model for human vaccine development, allowing both lymph node (LN) and circulating immune responses to be longitudinally sampled for correlates of vaccine efficacy. However, patterns of vaccine Ag drainage via the lymphatics after i.m. immunization can be stochastic, driving uneven deposition between lymphoid sites and between individual LN within larger clusters. To improve the accurate isolation of Ag-exposed LN during biopsies and necropsies, we developed and validated a method for coformulating candidate vaccines with tattoo ink i..
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Grants
Awarded by National Health and Medical Research Council
Awarded by European Union
Awarded by Australian Medical Research Future Fund
Awarded by European Union Horizon 2020 Research and Innovation Programme grant
Funding Acknowledgements
This work was supported by a National Health and Medical Research Council program grant (APP1149990), a European Union collaborative award (APP1115828), and fellowships (to S.J.K., A.K.W., and J.A.J.); the European Union Horizon 2020 Research and Innovation Programme grant 681137; the Victorian government; and the Australian Medical Research Future Fund (GNT2002073).