Journal article

Analysis of daily variation in the release of faecal eggs and coproantigen of Fasciola hepatica in naturally infected dairy cattle and the impact on diagnostic test sensitivity

Jane M Kelley, Mark A Stevenson, Vignesh Rathinasamy, Grant Rawlin, Travis Beddoe, Terry W Spithill



The liver fluke, Fasciola hepatica (F. hepatica) is a widespread parasite infection in dairy cattle in Victoria, South-eastern Australia. Robust diagnosis of fluke infection is needed in dairy cattle to identify sub-clinical infections which often go unnoticed, causing significant production losses. We tested the coproantigen ELISA (cELISA) and the FlukeFinder faecal egg count kit® on naturally infected cows in a fluke endemic region of Victoria. The aim of the study was to investigate the variation in the release of coproantigens and eggs into faeces over a 5-day period, at the morning (AM) and afternoon (PM) milkings, and to assess the impact of the timing of faecal sample collection on di..

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University of Melbourne Researchers


Funding Acknowledgements

The authors extend their thanks to Stewart and Nita McRae and the veterinarians and animal health officers employed by the State Government of Victoria. The authors would like to thank Jaclyn Swan for her assistance in processing the samples and Gillian Mitchell and Philip Skuce from the Moredun Research Institute, UK, for sequencing the paramphistome DNA. This work was supported by funds from the Gardiner Dairy Foundation, Dairy Australia, La Trobe University and the Victorian Department of Jobs, Precincts and Regions.