Journal article
Inoculation of barley with Trichoderma harzianum T-22 modifies lipids and metabolites to improve salt tolerance
S Gupta, PMC Smith, BA Boughton, TWT Rupasinghe, SHA Natera, U Roessner
Journal of Experimental Botany | OXFORD UNIV PRESS | Published : 2021
DOI: 10.1093/jxb/erab335
Abstract
Soil salinity has a serious impact on plant growth and agricultural yield. Inoculation of crop plants with fungal endophytes is a cost-effective way to improve salt tolerance. We used metabolomics to study how Trichoderma harzianum T-22 alleviates NaCl-induced stress in two barley (Hordeum vulgare L.) cultivars, Gairdner and Vlamingh, with contrasting salinity tolerance. GC-MS was used to analyse polar metabolites and LC-MS to analyse lipids in roots during the early stages of interaction with Trichoderma. Inoculation reversed the severe effects of salt on root length in sensitive cv. Gairdner and, to a lesser extent, improved root growth in more tolerance cv. Vlamingh. Biochemical changes s..
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Funding Acknowledgements
The authors would like to thank A/Prof Stuart Roy (University of Adelaide) for providing barley seeds.We also thank Himasha Mendes for her help and support for GC-MS analysis. Lipid and metabolite analyses were performed at Metabolomics Australia at the University of Melbourne, which is a National Collaborative Research Infrastructure Strategy initiative under Bioplatforms Australia Pty Ltd (http://www.bioplatforms.com/). S.G gratefully acknowledges the David Lachlan Hay Memorial Fund (The University of Melbourne) for financial support during the preparation of this article.