Journal article

Genomic surveillance of antimicrobial resistant bacterial colonisation and infection in intensive care patients

Kelly L Wyres, Jane Hawkey, Mirianne Mirceta, Louise M Judd, Ryan R Wick, Claire L Gorrie, Nigel F Pratt, Jill S Garlick, Kerrie M Watson, David V Pilcher, Steve A McGloughlin, Iain J Abbott, Nenad Macesic, Denis W Spelman, Adam WJ Jenney, Kathryn E Holt



BACKGROUND: Third-generation cephalosporin-resistant Gram-negatives (3GCR-GN) and vancomycin-resistant enterococci (VRE) are common causes of multi-drug resistant healthcare-associated infections, for which gut colonisation is considered a prerequisite. However, there remains a key knowledge gap about colonisation and infection dynamics in high-risk settings such as the intensive care unit (ICU), thus hampering infection prevention efforts. METHODS: We performed a three-month prospective genomic survey of infecting and gut-colonising 3GCR-GN and VRE among patients admitted to an Australian ICU. Bacteria were isolated from rectal swabs (n = 287 and n = 103 patients ≤2 and > 2 days from admiss..

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Awarded by National Health and Medical Research Council of Australia

Awarded by Bill & Melinda Gates Foundation

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council of Australia (Project Grant 1043822, Investigator Grant APP1176192 to KLW), the Viertel Charitable Foundation of Australia (Senior Medical Research Fellowship to KEH.) and this work was supported, in whole or in part, by the Bill & Melinda Gates Foundation (OPP1175797). Under the grant conditions of the Bill and Melinda Gates Foundation, a Creative Commons Attribution 4.0 Generic License has already been assigned to the Author Accepted Manuscript version that might arise from this submission. The funders played no role in the design of the study, the collection, analysis or interpretation of data, or writing the manuscript.