Journal article

Cell surface carbohydrates of symbiotic dinoflagellates and their role in the establishment of cnidarian-dinoflagellate symbiosis

Giada Tortorelli, Carsten Rautengarten, Antony Bacic, Gabriela Segal, Berit Ebert, Simon K Davy, Madeleine JH van Oppen, Geoffrey McFadden

ISME JOURNAL | SPRINGERNATURE | Published : 2021

Abstract

Symbiodiniaceae algae are often photosymbionts of reef-building corals. The establishment of their symbiosis resembles a microbial infection where eukaryotic pattern recognition receptors (e.g. lectins) are thought to recognize a specific range of taxon-specific microbial-associated molecular patterns (e.g. glycans). The present study used the sea anemone, Exaiptasia diaphana and three species of Symbiodiniaceae (the homologous Breviolum minutum, the heterologous-compatible Cladocopium goreaui and the heterologous-incompatible Fugacium kawagutii) to compare the surface glycomes of three symbionts and explore the role of glycan-lectin interactions in host-symbiont recognition and establishmen..

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Grants

Awarded by Australian Government through the Australian Research Council


Awarded by Australian Research Council


Awarded by Australian Research Council Laureate Fellowship


Awarded by ARC


Funding Acknowledgements

This research was funded by the Australian Government through the Australian Research Council Discovery Project DP160101539 and DP210100639. GT acknowledges receipt of the University of Melbourne International Research Scholarship and Fee Remission Scholarship, and the Botany Foundation at the University of Melbourne for awarding the Ethel McLennan prize that supported Symbiodiniaceae monosaccharide and lectin array analyses. GIMcF and MJHvO acknowledge Australian Research Council Discovery Grant DP160101539. MJHvO acknowledges Australian Research Council Laureate Fellowship FL180100036. BE acknowledges an ARC Future Fellowship Award FT160100276, Discovery Grant DP180102630 and support from the University of Melbourne Botany Foundation. CR acknowledges the financial aid of an Albert Shimmins COVID-19 support fund. We thank Joshua Heazlewood and Alfie Hao for the sugar nucleotide and the monosaccharide component analyses. We thank Virginia Weis, John Parkinson, Paige Mandelare and Kruti Patel for the support in the preparation of lectin array samples. We thank Ellie Cho from the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne for assistance with confocal microscopy. We thank Kevin Bairos-Novak, Patrick Buerger and Wing Chan for the help with bioinformatics.