Journal article

A haploid diamondback moth (Plutella xylostella L.) genome assembly resolves 31 chromosomes and identifies a diamide resistance mutation

CM Ward, KD Perry, G Baker, K Powis, DG Heckel, SW Baxter

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021

Abstract

The diamondback moth, Plutella xylostella (L.), is a highly mobile brassica crop pest with worldwide distribution and can rapidly evolve resistance to insecticides, including group 28 diamides. Reference genomes assembled using Illumina sequencing technology have provided valuable resources to advance our knowledge regarding the biology, origin and movement of diamondback moth, and more recently with its sister species, Plutella australiana. Here we apply a trio binning approach to sequence and annotate a chromosome level reference genome of P. xylostella using PacBio Sequel and Dovetail Hi-C sequencing technology and identify a point mutation that causes resistance to commercial diamides. A..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Awarded by Grains Research and Development Corporation


Funding Acknowledgements

This research was supported by the Australian Research Council (Grant FT140101303) , the Grains Research and Development Corpora-tion (Grant 9175870) and by the Max-Planck-Gesellschaft.