The Hippo pathway uses different machinery to control cell fate and organ size

Jonathan M Pojer; Samuel A Manning; Benjamin Kroeger; Shu Kondo; Kieran F Harvey

Journal article

The Hippo pathway uses different machinery to control cell fate and organ size

Jonathan M Pojer, Samuel A Manning, Benjamin Kroeger, Shu Kondo, Kieran F Harvey

ISCIENCE | CELL PRESS | Published : 2021

DOI: 10.1016/j.isci.2021.102830

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Abstract

The Hippo pathway is a conserved signaling network that regulates organ growth and cell fate. One such cell fate decision is that of R8 photoreceptor cells in the Drosophila eye, where Hippo specifies whether cells sense blue or green light. We show that only a subset of proteins that control organ growth via the Hippo pathway also regulate R8 cell fate choice, including the STRIPAK complex, Tao, Pez, and 14-3-3 proteins. Furthermore, key Hippo pathway proteins were primarily cytoplasmic in R8 cells rather than localized to specific membrane domains, as in cells of growing epithelial organs. Additionally, Warts was the only Hippo pathway protein to be differentially expressed between R8 subt..

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University of Melbourne Researchers

Kieran Harvey Author The Sir Peter Maccallum Department of Oncology

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian Research Council

Awarded by NHMRC

Funding Acknowledgements

We thank C. Desplan for comments on the manuscript and reagents and R. Johnston, the Bloomington Drosophila Stock Center, the Vienna Drosophila RNAi Center, the Australian Drosophila Research Support Facility (www.ozdros.com), and the Developmental Studies Hybridoma Bank for D. melanogaster stocks and antibodies. K.F.H was supported by a Senior Research Fellowship (APP1078220) and Investigator grant (APP1194467) from the National Health and Medical Research Council (NHMRC) and J. M.P. was supported by an Australian Postgraduate Award. This research was supported by the Australian Research Council (DP180102044) and the NHMRC (APP1157737). We acknowledge the Peter Mac Centre for Advanced Histology andMicroscopy and support to themfromthe PeterMacCallumCancer Foundation and the Australian Cancer Research Foundation.