Journal article

Spatially Fractionated X-Ray Microbeams Elicit a More Sustained Immune and Inflammatory Response in the Brainstem than Homogenous Irradiation

Lloyd ML Smyth, Jeffrey C Crosbie, Clare Sloggett, Peter AW Rogers, Jacqueline F Donoghue



Synchrotron microbeam radiation therapy (MRT) is a preclinical irradiation technique which could be used to treat intracranial malignancies. The goal of this work was to discern differences in gene expression and the predicted regulation of molecular pathways in the brainstem after MRT versus synchrotron broad-beam radiation therapy (SBBR). Healthy C57BL/6 mice received whole-head irradiation with median acute toxic doses of MRT (241 Gy peak dose) or SBBR (13 Gy). Brains were harvested 4 and 48 h postirradiation and RNA was extracted from the brainstem. RNA-sequencing was performed to identify differentially expressed genes (false discovery rate < 0.01) relative to nonirradiated controls and..

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Funding Acknowledgements

This work was supported by a research grant from the Victorian Medical Radiation Practitioners Education Trust. LS was supported by a Research Training Program scholarship from the Australian Government. We thank Matthew Tinning and the team at the Australian Genome Research Facility for performing RNA-sequencing and the Australian Nuclear Science and Technology Organisation for beam-time and technical support at the Australian Synchrotron Imaging and Medical Beamline.