Journal article

STUDY ON THE IN VITRO MORPHOGENETIC RESPONSE FROM SHOOT AND CALLUS CULTURES OF Blepharis maderaspatensis (L.) Heyne ex. Roth

Trang Phuong Nguyen Thi, Quang Minh Bui, Hai Duc Le, Linh Quoc Nguyen

The Scientific Journal of Tra Vinh University | Tra Vinh University

Abstract

Blepharis maderaspatensis (L.) Heyne ex. Roth is a short-term plant which contains many important secondarycompounds with high medicinal value. Currently, most of the researches focus on chemical composition and pharmacological activity, but the source of raw materials is very limited. In this study, the first step is transferring the samples from nature into in vitro culture conditions to understand the effects of the factors related to shooting and callus morphogenesis was performed, the first node from shoots apical meristem was isolated and sterilized with 1.5% NaOCl for 20 minutes to achieve high efficiency with 86.11% sterile samples and 85.56% shoot growth rate after 2 weeks of cultur..

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