Loss of the D beta 1 nicotinic acetylcholine receptor subunit disrupts bursicon-driven wing expansion and diminishes adult viability in Drosophila melanogaster
Danielle Christesen, Ying Ting Yang, Wei Chen, Philip Batterham, Trent Perry
GENETICS | OXFORD UNIV PRESS INC | Published : 2021
Abstract Cholinergic signaling dominates the insect central nervous system, contributing to numerous fundamental pathways and behavioral circuits. However, we are only just beginning to uncover the diverse roles different cholinergic receptors may play. Historically, insect nicotinic acetylcholine receptors have received attention due to several subunits being key insecticide targets. More recently, there has been a focus on teasing apart the roles of these receptors, and their constituent subunits, in native signaling pathways. In this study, we use CRISPR-Cas9 genome editing to generate germline and somatic deletions of the Dβ1 nicotinic acetylcholine receptor subunit and in..View full abstract
Awarded by Australian Research Council
Funding was provided through an Australian Research Council Discovery Project awarded to P.B. (DP160100332), and an Australian Postgraduate Award, and Dame Margaret Blackwood Soroptimist Scholarship awarded to D.C.