Journal article

IFITM Proteins That Restrict the Early Stages of Respiratory Virus Infection Do Not Influence Late-Stage Replication

Tina Meischel, Svenja Fritzlar, Fernando Villalon-Letelier, Melkamu B Tessema, Andrew G Brooks, Patrick C Reading, Sarah L Londrigan



Interferon-induced transmembrane (IFITM) proteins inhibit a broad range of enveloped viruses by blocking entry into host cells. We used an inducible overexpression system to investigate if IFITM1, IFITM2, and IFITM3 could modulate early and/or late stages of influenza A virus (IAV) or parainfluenza virus 3 (PIV-3) infection in human A549 airway epithelial cells. IAV and PIV-3 represent respiratory viruses which utilize distinct cellular entry pathways. We verify entry by endocytosis for IAV, whereas PIV-3 infection was consistent with fusion at the plasma membrane. Following induction prior to infection, all three IFITM proteins restricted the percentage of IAV-infected cells at 8 hours post..

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Awarded by National Health and Medical Research Council (NHMRC) of Australia

Funding Acknowledgements

This study was supported by project grants APP1143154, APP1083307, and APP1184532 from the National Health and Medical Research Council (NHMRC) of Australia. P.C. R., S.L.L., and A.G.B. are all recipients of funding from the NHMRC. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.