Journal article
Multimodal regulation of encystation in Giardia duodenalis revealed by deep proteomics
B Balan, SJ Emery-Corbin, JJ Sandow, BRE Ansell, S Tichkule, AI Webb, SG Svärd, AR Jex
International Journal for Parasitology | ELSEVIER SCI LTD | Published : 2021
Open access
Abstract
Cyst formation in the parasitic protist Giardia duodenalis is critical to its transmission. Existing proteomic data quantifies only 17% of coding genes transcribed during encystation and does not cover the complete process from trophozoite to mature cyst. Using high-resolution mass spectrometry, we have quantified proteomic changes across encystation and compared this with published transcriptomic data. We reproducibly identified 3863 (64.5% of Giardia proteins) and quantified 3382 proteins (56.5% of Giardia proteins) over standard trophozoite growth (TY), during low-bile encystation priming (LB), 16 h into encystation (EC), and at cyst maturation (C). This work provides the first known expa..
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Awarded by Jack Brockhoff Foundation
Funding Acknowledgements
B. Balan's PhD scholarship was funded by Melbourne International Research Scholarship, University of Melbourne, Australia. We gratefully acknowledge the Walter and Eliza Hall Research Institute, Australia, for the top up scholarship. AR Jex acknowledges the Australian National Health and Medical Research Foundation Career Development Fellowship program (APP1126395). SJ Emery-Corbin was funded by a Jack Brockhoff Foundation (Australia) Early Career Grant (ID JBF 4184, 2016). We also acknowledge funding from the Victorian State Government Operational Infrastructure Support, Australia and the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. Proteomics analysis was performed at the Mass Spectrometry and Proteomics Facility, Bio21 Institute, University of Melbourne. We also acknowledge Dr. Chin Seng Ang (Mass Spectrometry and Proteomics Facility, Bio21 Institute, University of Melbourne) for providing technical support. We also thank Amrita Vijay for all the expert opinions given regarding this manuscript.