Journal article

Spatial omics and multiplexed imaging to explore cancer biology

Sabrina M Lewis, Marie-Liesse Asselin-Labat, Nguyen Quan, Jean Berthelet, Xiao Tan, Verena C Wimmer, Delphine Merino, Kelly L Rogers, Shalin H Naik

NATURE METHODS | NATURE PORTFOLIO | Published : 2021

Abstract

Understanding intratumoral heterogeneity-the molecular variation among cells within a tumor-promises to address outstanding questions in cancer biology and improve the diagnosis and treatment of specific cancer subtypes. Single-cell analyses, especially RNA sequencing and other genomics modalities, have been transformative in revealing novel biomarkers and molecular regulators associated with tumor growth, metastasis and drug resistance. However, these approaches fail to provide a complete picture of tumor biology, as information on cellular location within the tumor microenvironment is lost. New technologies leveraging multiplexed fluorescence, DNA, RNA and isotope labeling enable the detec..

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University of Melbourne Researchers

Grants

Awarded by NHMRC


Awarded by Australian Research Council DECRA fellowship


Awarded by Susan G. Komen and Cancer Australia


Awarded by National Breast Cancer Foundation, Australia


Funding Acknowledgements

For Fig. 2, we thank L. Cai for contributing seqFISH data; S. Lakhani and P. Kalita de Croft for clinical annotation of breast cancer tissue; and Ionpath for assisting with tissue staining and generation of MIBI data. We thank D. Brown and T. Weber for critical feedback. M.-L.A.-L. is supported by funding from the Viertel Foundation Senior Medical Research Fellowship, NHMRC grant GNT1182155 and the Harry Secomb Foundation, managed by Perpetual. Q.N. is supported by Australian Research Council DECRA fellowship DE190100116 and NHMRC grant GNT2001514. D.M. is supported by Susan G. Komen and Cancer Australia, CCR19606878, a grant from the National Breast Cancer Foundation, Australia, IIRS-19-082 and the Grant-in-Aid Scheme administered by Cancer Council Victoria. The Olivia Newton-John Cancer Research Institute gratefully acknowledges the generous support of the Love Your Sister Foundation. S.H.N. is supported by NHMRC grants GNT1062820, GNT1100033, GNT1101378, GNT1124812 and GNT1145184. We also acknowledge support from the ACRF Centre for Imaging the Tumour Environment at the Olivia Newton-John Cancer Research Institute, the ACRF Program for Resolving Cancer Complexity and Therapeutic Resistance at WEHI and the Operational Infrastructure Support Program provided by the Victorian government and the NHMRC Independent Research Institutes Infrastructure Support Scheme (IRIISS) grant. The contents of the published material are solely the responsibility of the individual authors and do not reflect the views of Cancer Australia, NHMRC or other funding agencies.