Development and validation of a new method to isolate, expand, and differentiate circulating osteogenic precursor (COP) cells
Jack Feehan, Kulmira Nurgali, Vasso Apostolopoulos, Gustavo Duque
BONE REPORTS | ELSEVIER | Published : 2021
Circulating osteogenic precursor (COP) cells are a population of progenitor cells in the peripheral blood with the capacity to form bone in vitro and in vivo. They have characteristics of the mesenchymal stem and progenitor pool found in the bone marrow; however, more recently, a population of COP cells has been identified with markers of the hematopoietic lineage such as CD45 and CD34. While this population has been associated with several bone pathologies, a lack of cell culture models and inconsistent characterization has limited mechanistic research into their behavior and physiology. In this study, we describe a method for the isolation of CD45+/CD34+/alkaline phosphatase (ALP) + COP ce..View full abstract
This project was funded via a Seed Grant from the Australian Institute for Musculoskeletal Science (AIMSS).