Journal article

The post-translational modification landscape of commercial beers

Edward D Kerr, Christopher H Caboche, Cassandra L Pegg, Toan K Phung, Claudia Gonzalez Viejo, Sigfredo Fuentes, Mark T Howes, Kate Howell, Benjamin L Schulz



Beer is one of the most popular beverages worldwide. As a product of variable agricultural ingredients and processes, beer has high molecular complexity. We used DIA/SWATH-MS to investigate the proteomic complexity and diversity of 23 commercial Australian beers. While the overall complexity of the beer proteome was modest, with contributions from barley and yeast proteins, we uncovered a very high diversity of post-translational modifications (PTMs), especially proteolysis, glycation, and glycosylation. Proteolysis was widespread throughout barley proteins, but showed clear site-specificity. Oligohexose modifications were common on lysines in barley proteins, consistent with glycation by ma..

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Funding Acknowledgements

We thank Dr Amanda Nouwens and Peter Josh at The University of Queensland, School of Chemistry and Molecular Biosciences Mass Spectrometry Facility for their assistance and expertise. Edward D. Kerr was funded by an Advance Queensland PhD Scholarship. Christopher H. Caboche was funded by a Wine Australia PhD Scholarship.