An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
Hao Deng, Asanka Jayawardena, Jianxiong Chan, Sher Maine Tan, Tuncay Alan, Patrick Kwan
SCIENTIFIC REPORTS | NATURE PORTFOLIO | Published : 2021
There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm3) unit. The test is easy to operate and does not r..View full abstract
Awarded by Medical Research Future Fund Fellowship
This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). P.K. is supported by a Medical Research Future Fund Fellowship (MRF113642).