Journal article

An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection

Hao Deng, Asanka Jayawardena, Jianxiong Chan, Sher Maine Tan, Tuncay Alan, Patrick Kwan

SCIENTIFIC REPORTS | NATURE PORTFOLIO | Published : 2021

Abstract

There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm3) unit. The test is easy to operate and does not r..

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