Journal article

Protease-activated receptor-2 promotes osteogenesis in skeletal mesenchymal stem cells at the expense of adipogenesis: Involvement of interleukin-6

R Sanaei, PK Kularathna, N Taghavi, JD Hooper, CN Pagel, EJ Mackie

BONE REPORTS | ELSEVIER | Published : 2021


Bone marrow mesenchymal stem cells (MSCs) give rise to osteoblasts and adipocytes, with an inverse relationship between the two. The MSCs from protease-activated receptor-2 knockout (PAR2 KO) mice have a reduced capacity to generate osteoblasts. Here we describe the observation that PAR2 KO osteoblastic cultures generate more adipocytes than wildtype (WT) cultures. Osteoblasts from PAR2 KO mice expressed lower levels of osteoblastic genes (Runx2, Col1a1 and Bglap), and higher levels of the adipocytic gene Pparg than WT osteoblasts. Bone marrow stromal cells from PAR2 KO mice generated fewer osteoblastic colonies (assessed by staining for alkaline phosphatase activity and mineral deposition) ..

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Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

Funding was provided by the National Health and Medical Research Council of Australia (Project grant no. 614206). The funding source had no involvement in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.RS and NT were each supported by an International Postgraduate Research Scholarship and an Australian Postgraduate Award, and PK was supported by scholarships from the University of Melbourne.