Guiding the Immune Response to a Conserved Epitope in MSP2, an Intrinsically Disordered Malaria Vaccine Candidate
Jeffrey Seow, Sreedam C Das, Rodrigo AV Morales, Ricardo Ataide, Bankala Krishnarjuna, Mitchell Silk, David K Chalmers, Jack Richards, Robin F Anders, Christopher A MacRaild, Raymond S Norton
VACCINES | MDPI | Published : 2021
The malaria vaccine candidate merozoite surface protein 2 (MSP2) has shown promise in clinical trials and is in part responsible for a reduction in parasite densities. However, strain-specific reductions in parasitaemia suggested that polymorphic regions of MSP2 are immuno-dominant. One strategy to bypass the hurdle of strain-specificity is to bias the immune response towards the conserved regions. Two mouse monoclonal antibodies, 4D11 and 9H4, recognise the conserved C-terminal region of MSP2. Although they bind overlapping epitopes, 4D11 reacts more strongly with native MSP2, suggesting that its epitope is more accessible on the parasite surface. In this study, a structure-based vaccine de..View full abstract
Awarded by National Health and Medical Research Council of Australia
This research was funded in part by the National Health and Medical Research Council of Australia (project grant 1042520 and fellowships to J.S.R. and R.S.N.) and in part by an Australian Government Research Training Program Scholarship (to J.S.).