Journal article
Profiling the glycome of Cardicola forsteri, a blood fluke parasitic to bluefin tuna
L Coff, JL Abrahams, S Collett, C Power, BF Nowak, D Kolarich, NJ Bott, PA Ramsland
International Journal for Parasitology | Published : 2022
Abstract
Infections by blood flukes (Cardicola spp.) are considered the most significant health issue for ranched bluefin tuna, a major aquaculture industry in Japan and Australia. The host–parasite interfaces of trematodes, namely their teguments, are particularly rich in carbohydrates, which function both in evasion and modulation of the host immune system, while some are primary antigenic targets. In this study, histochemistry and mass spectrometry techniques were used to profile the glycans of Cardicola forsteri. Fluorescent lectin staining of adult flukes indicates the presence of oligomannose (Concanavalin A-reactive) and fucosylated (Pisum sativum agglutinin-reactive) N-glycans. Additionally, ..
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Awarded by Australian Government
Funding Acknowledgements
We thank the Australian Southern Bluefin Tuna Industry Associa-tion and commercial tuna companies for their assistance and sup-port in sample collection. We would like to acknowledge the assistance and support of Dr. Andreia Almeida and Tiago Oliveira (Griffith University, Australia) with sample preparation and pro-cessing for mass spectrometry. This work was financially supported by the Australian Government through the Fisheries Research and Development Corporation (FRDC 2017-241 and 2018-170) ; RMIT University, Australia (CDF17132) ; and the Aus-tralian Society for Parasitology (ASPTA_LC2019) . LC, SC, and CP are supported by a Research Training Program stipend scholarship from the Australian Government, Department of Education and Training. DK is the recipient of an Australian Research Council Future Fellowship (FT160100344) , funded by the Australian Government. The funding bodies were not involved in the design of the study, nor in the collection, analysis, and interpretation of the data, nor in the writing and submission of the manuscript for publication.