Journal article

The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs

Lung-Yu Liang, Michael Roy, Christopher R Horne, Jarrod J Sandow, Minglyanna Surudoi, Laura F Dagley, Samuel N Young, Toby Dite, Jeffrey J Babon, Peter W Janes, Onisha Patel, James M Murphy, Isabelle S Lucet

BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 2021

Abstract

EphB6 and EphA10 are two poorly characterised pseudokinase members of the Eph receptor family, which collectively serves as mediators of contact-dependent cell-cell communication to transmit extracellular cues into intracellular signals. As per their active counterparts, EphB6 and EphA10 deregulation is strongly linked to proliferative diseases. However, unlike active Eph receptors, whose catalytic activities are thought to initiate an intracellular signalling cascade, EphB6 and EphA10 are classified as catalytically dead, raising the question of how non-catalytic functions contribute to Eph receptor signalling homeostasis. In this study, we have characterised the biochemical properties and ..

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Grants

Awarded by NHMRC


Awarded by NHMRC IRIISS


Funding Acknowledgements

L.-Y.L. was supported by a Melbourne Research Scholarship. Additional support was received from the Australian Cancer Research Foundation (to I.S.L., L.-Y.L., O.P., M.S., M.R.). I.S.L. acknowledges support from the Walter and Eliza Hall Institute. J.M.M. is grateful to the NHMRC for fellowship support (1172929). We acknowledge the NHMRC IRIISS (9000653) and the Victorian State Government Operational Infrastructure Support Scheme. We thank the staff of the Australian Synchrotron SAXS/WAXS beamline for assistance with data collection.