Journal article

Diagnostic accuracy of a novel enzyme-linked immunoassay for the detection of IgG and IgG4 against Strongyloides stercoralis based on the recombinant antigens NIE/SsIR

Francesca Tamarozzi, Silvia Stefania Longoni, Cristina Mazzi, Sofia Pettene, Antonio Montresor, Siddhartha Mahanty, Zeno Bisoffi, Dora Buonfrate

PARASITES & VECTORS | BMC | Published : 2021

Abstract

BACKGROUND: The diagnosis of strongyloidiasis is challenging. Serological tests are acknowledged to have high sensitivity, but issues due to cross-reactions with other parasites, native parasite antigen supply and intrinsic test variability do occur. Assays based on recombinant antigens could represent an improvement. The aim of this study was to assess the sensitivity and specificity of two novel immunoglobulin (Ig)G and IgG4 enzyme-linked immunosorbent assays (ELISAs) based on the recombinant antigens NIE/SsIR for the diagnosis of strongyloidiasis. METHODS: This was a retrospective diagnostic accuracy study. We included serum samples collected from immigrants from strongyloidiasis endemic ..

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University of Melbourne Researchers

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Funding Acknowledgements

This work was partly funded by the Italian Ministry of Health "Fondi Ricerca Corrente-L2P4" to IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy. The Strongy DetectT IgG ELISA and IgG4 ELISA kits were donated by the Manufaturer, InBios International Inc, Seattle, WA, USA.