Journal article

SARS-CoV-2 suppresses IFNβ production mediated by NSP1, 5, 6, 15, ORF6 and ORF7b but does not suppress the effects of added interferon.

Maya Shemesh, Turgut E Aktepe, Joshua M Deerain, Julie L McAuley, Michelle D Audsley, Cassandra T David, Damian FJ Purcell, Victoria Urin, Rune Hartmann, Gregory W Moseley, Jason M Mackenzie, Gideon Schreiber, Daniel Harari

PLoS Pathog | Published : 2021

Abstract

Type I Interferons (IFN-Is) are a family of cytokines which play a major role in inhibiting viral infection. Resultantly, many viruses have evolved mechanisms in which to evade the IFN-I response. Here we tested the impact of expression of 27 different SARS-CoV-2 genes in relation to their effect on IFN production and activity using three independent experimental methods. We identified six gene products; NSP6, ORF6, ORF7b, NSP1, NSP5 and NSP15, which strongly (>10-fold) blocked MAVS-induced (but not TRIF-induced) IFNβ production. Expression of the first three of these SARS-CoV-2 genes specifically blocked MAVS-induced IFNβ-promoter activity, whereas all six genes induced a collapse in IFNβ m..

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