Coordinated action of multiple transporters in the acquisition of essential cationic amino acids by the intracellular parasite Toxoplasma gondii
Stephen J Fairweather, Esther Rajendran, Martin Blume, Kiran Javed, Birte Steinhofel, Malcolm J McConville, Kiaran Kirk, Stefan Broer, Giel G van Dooren
PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2021
Intracellular parasites of the phylum Apicomplexa are dependent on the scavenging of essential amino acids from their hosts. We previously identified a large family of apicomplexan-specific plasma membrane-localized amino acid transporters, the ApiATs, and showed that the Toxoplasma gondii transporter TgApiAT1 functions in the selective uptake of arginine. TgApiAT1 is essential for parasite virulence, but dispensable for parasite growth in medium containing high concentrations of arginine, indicating the presence of at least one other arginine transporter. Here we identify TgApiAT6-1 as the second arginine transporter. Using a combination of parasite assays and heterologous characterisation ..View full abstract
Awarded by Australian Research Council
This work was supported by Discovery Grants from the Australian Research Council to KK, GGvD and SB (DP150102883) and to GGvD and KK (DP200100483). MJM is a NHMRC Principal Research Fellow (APP1154540). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.