Journal article
VEGF, FGF2, and BMP4 regulate transitions of mesoderm to endothelium and blood cells in a human model of yolk sac hematopoiesis
FF Bruveris, ES Ng, EG Stanley, AG Elefanty
Experimental Hematology | Published : 2021
Abstract
Exogenous growth factors play an important role in mediating hematopoietic differentiation of human pluripotent stem cells. We explored the role of different factors in early human blood cell production using blast colony formation in methylcellulose as a surrogate assay for yolk sac hematopoiesis. A reporter cell line that read out endothelial (SOX17+) and hematopoietic (RUNX1C+) progenitors facilitated the identification of basic fibroblast growth and vascular endothelial growth factor as critical signals for the progression of mesoderm into endothelium. Bone morphogenetic protein 4 was needed for the subsequent generation of blood from hemogenic endothelium, and this was antagonized by Ac..
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Awarded by English Goethe Society
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia through research fellowships awarded to AGE (GNT1117596) and EGS (GNT1079004) and project grants awarded to AG Elefanty and EG Stanley (GNT1068866, GNT1129861), by the Australian Research Council Special Research Initiative in Stem Cells (Stem Cells Australia) and by the Stafford Fox Medical Research Foundation. Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian Government's Operational Infrastructure Support Program.