The use of SUnSET as a non-radioactive method for measuring rates of protein synthesis in whole skeletal muscles and in single fibers

Abstract

SUnSET (SUrface SEnsing of Translation) involves the immunological detection of puromycin in nascent peptides as a means for quantifying rates of protein synthesis (PS). To validate SUnSET against an established technique, plantaris muscles were subjected to synergist ablation (SA) or sham surgery. After 7d, muscles were incubated ex vivo with a flooding dose of 3 H‐Phenylalanine ( 3 H‐P) or with puromycin. According to the established 3 H‐P method, SA induced a 3.4 fold increase in PS while a western blot (WB) version of SUnSET revea..