Elucidating the Motif for CpG Oligonucleotide Binding to the Dendritic Cell Receptor DEC-205 Leads to Improved Adjuvants for Liver-Resident Memory.
Jessica Li, Fatma Panetta, Meredith O'Keeffe, Ingrid M Leal Rojas, Kristen J Radford, Jian-Guo Zhang, Daniel Fernandez-Ruiz, Gayle M Davey, Benjamin S Gully, Kirsteen M Tullett, Jamie Rossjohn, Richard Berry, Chin-Nien Lee, Mireille H Lahoud, William R Heath, Irina Caminschi
J Immunol | The American Association of Immunologists | Published : 2021
DEC-205 is a cell-surface receptor that transports bound ligands into the endocytic pathway for degradation or release within lysosomal endosomes. This receptor has been reported to bind a number of ligands, including keratin, and some classes of CpG oligodeoxynucleotides (ODN). In this study, we explore in detail the requirements for binding ODNs, revealing that DEC-205 efficiently binds single-stranded, phosphorothioated ODN of ≥14 bases, with preference for the DNA base thymidine, but with no requirement for a CpG motif. DEC-205 fails to bind double-stranded phosphodiester ODN, and thus does not bind the natural type of DNA found in mammals. The ODN binding preferences of DEC-205 result i..View full abstract