Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity.
Wuji Zhang, Brendon Chua, Kevin Selva, Lukasz Kedzierski, Thomas Ashhurst, Ebene Haycroft, Suzanne Shoffner, Luca Hensen, David Boyd, Fiona James, Effie Mouhtouris, Jason Kwong, Kyra Chua, George Drewett, Ana Copaescu, Julie Dobson, Louise Rowntree, Jennifer Habel, Lilith Allen, Hui-Fern Koay Show all
Published : 2021
Although the respiratory tract is the primary site of SARS-CoV-2 infection and the ensuing immunopathology, respiratory immune responses are understudied and urgently needed to understand mechanisms underlying COVID-19 disease pathogenesis. We collected paired longitudinal blood and respiratory tract samples (endotracheal aspirate, sputum or pleural fluid) from hospitalized COVID-19 patients and non-COVID-19 controls. Cellular, humoral and cytokine responses were analysed and correlated with clinical data. SARS-CoV-2-specific IgM, IgG and IgA antibodies were detected using ELISA and multiplex assay in both the respiratory tract and blood of COVID-19 patients, although a higher receptor bindi..View full abstract