Journal article

Identification of in vivo expressed proteins in live attenuated lipopolysaccharide mutant that mediates heterologous protection against Leptospira spp

Theerapat Phoka, Teerasit Techawiwattanaboon, Noppadon Sangjun, Pat Komanee, Gerald L Murray, Rasana Wongratanacheewin Sermswan, Ben Adler, Kanitha Patarakul



Leptospirosis vaccines that elicit broad protection against a range of pathogenic Leptospira spp. would overcome a major drawback of currently licensed bacterin vaccines. Live attenuated vaccine produced from a lipopolysaccharide (LPS) mutant strain of L. interrogans serovar Manilae M1352 (Live M1352) stimulated better protective efficacy than heat killed M1352 (HK M1352) against a heterologous challenge with L. interrogans serovar Pomona. To identify antigens of Live M1352 potentially responsible for cross protection, in vivo-induced antigen technology (IVIAT), a powerful tool to identify in vivo-induced (ivi) genes expressed during infection, was employed in this study. Pooled sera from ha..

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Awarded by Chulalongkorn University

Funding Acknowledgements

This study was financially supported by the 100th Anniversary Chulalongkorn University for Doctoral Scholarship and the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund), and partially funded by Chulalongkorn UniversityGB-CU-61-12-30-02. Work at Monash University was supported by grants from the National Health and Medical Research Council and the Australian Research Council.