Journal article
Interleukin-10 conjugated electrospun polycaprolactone (PCL) nanofibre scaffolds for promoting alternatively activated (M2) macrophages around the peripheral nerve in vivo
JR Potas, F Haque, FL Maclean, DR Nisbet
Journal of Immunological Methods | Published : 2015
Abstract
Macrophages play a key role in tissue regeneration following peripheral nerve injury by preparing the surrounding parenchyma for regeneration, however, they can be damaging if the response is excessive. Interleukin 10 (IL-10) is a cytokine that promotes macrophages toward an anti-inflammatory/wound healing state (M2 phenotype). The bioactive half-life of IL-10 is dependent on the cellular microenvironment and ranges from minutes to hours in vivo. Our objective was to extend the in vivo bioavailability and bioactivity of IL-10 by attaching the protein onto nanofibrous scaffolds and demonstrating increased expression levels of M2 macrophages when placed around healthy intact peripheral nerves...
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
We thank Alexandra Rodriguez and Di Hu for training relating to the IL-10 attachment procedure and immunohistochemistry respectively. Kiara Bruggeman, Alexandra Rodriguez and Shayanti Mukherjee are thanked for critically reading the manuscript. This project was funded by the Bootes Foundation and National Health and Medical Research Council (APP1020332). David Nisbet was supported by an Australian Research Council Australian Postdoctoral Fellowship and subsequently by a NHMRC Career Development Fellowship (APP1050684). Some material components of the project were funded by the ARC (DP130103131). Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is also gratefully acknowledged.