Colonization and maintenance of murine embryonic stem cells on poly(α-hydroxy esters)

Abstract

The aim of this study was to determine the ability of various poly(α-hydroxy esters) to support the in vitro propagation of murine embryonic stem (ES) cells in an undifferentiated state. To this end, ES cell colonization, growth and Oct-4 immunoreactivity following a 48h culture period upon poly(D,L-lactide), poly(L-lactide), poly(glycolide) and poly(D,L-lactide-co-glycolide) (PLGA) were assessed. By the analysis of live and dead cell number indices and Oct-4 immunoreactivity, ES cell colonization rate during a 48h culture period was found to be significantly greater on PLGA compared to all the other unmodified poly(α-hydroxy esters) tested. Surface treatment of all polymers with 0.1M potass..