Journal article
Best practices for spatial profiling for breast cancer research with the GeoMx® digital spatial profiler
H Bergholtz, JM Carter, A Cesano, MCU Cheang, SE Church, P Divakar, CA Fuhrman, S Goel, J Gong, JL Guerriero, ML Hoang, ES Hwang, H Kuasne, J Lee, Y Liang, EA Mittendorf, J Perez, A Prat, L Pusztai, JW Reeves Show all
Cancers | Published : 2021
Abstract
Breast cancer is a heterogenous disease with variability in tumor cells and in the surrounding tumor microenvironment (TME). Understanding the molecular diversity in breast cancer is critical for improving prediction of therapeutic response and prognostication. High-plex spatial profiling of tumors enables characterization of heterogeneity in the breast TME, which can holistically illuminate the biology of tumor growth, dissemination and, ultimately, response to therapy. The GeoMx Digital Spatial Profiler (DSP) enables researchers to spatially resolve and quantify proteins and RNA transcripts from tissue sections. The platform is compatible with both formalin-fixed paraffin-embedded and froz..
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Funding Acknowledgements
The preparation of this manuscript was funded by NanoString Technologies Inc. as a salary of the co-authors from NanoString.