Journal article
Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
Y Hirokawa, J Clarke, M Palmieri, T Tan, D Mouradov, S Li, C Lin, F Li, H Luo, K Wu, M Faux, CW Tan, M Lee, G Gard, P Gibbs, AW Burgess, OM Sieber
Communications Biology | Published : 2021
Abstract
Cell embedment into a solid support matrix is considered essential for the culture of intestinal epithelial organoids and tumoroids, but this technique presents challenges that impede scalable culture expansion, experimental manipulation, high-throughput screening and diagnostic applications. We have developed a low-viscosity matrix (LVM) suspension culture method that enables efficient establishment and propagation of organoids and tumoroids from the human large intestine. Organoids and tumoroids cultured in LVM suspension recapitulate the morphological development observed in solid matrices, with tumoroids reflecting the histological features and genetic heterogeneity of primary colorectal..
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Awarded by Beijing Institute of Genomics, Chinese Academy of Sciences
Funding Acknowledgements
This study was supported by Cure Cancer Australia/Cancer Australia through a Priority-driven Collaborative Cancer Research Scheme Grant (APP1102534), a NHMRC Program Grant (APP1092788), a Beijing Genomics Institute (BGI) Genome Decode Program Grant, the Stafford Fox Medical Research Foundation, donations from Mr Jeff Cuff on behalf of the Shirley Cuff Cancer Research Foundation and Mr Shane Murphy, an Independent Research Institutes Infrastructure Support Scheme Grant and a Victorian State Government Operational Infrastructure Support Grant. O.M.S. is a National Health and Medical Research Council (NHMRC) Senior Research Fellow (APP1136119). This work was supported by the Guangdong Enterprise Key Laboratory of Human Disease Genomics (2020B1212070028), and by the China National GeneBank with computational and sequencing resources. The authors thank the Victorian Cancer BioBank and Biogrid Australia for provision of patient specimens and clinical data. We thank Dr Kelly Rogers and Cindy Evelyn from the WEHI Centre for Dynamic Imaging for assistance with imaging, Dr Kym Lowes, Dr Amanda Au and Dr Timothy Blackmore from the WEHI National Drug Discovery Centre for assistance with assay automation, and AProf David Williams from the Olivia Newton-John Cancer Research Institute for review of tissue and organoid histology.