Journal article
Spawning induction of the high-value white teatfish sea cucumber, Holothuria fuscogilva, using recombinant relaxin-like gonad stimulating peptide (RGP)
J Nocillado, NDQ Duy, HD Chieu, L Turner, RAD Bathgate, T Wang, MA Hossain, NV Hung, NH Ninh, SF Cummins, A Elizur
Aquaculture | Published : 2022
Abstract
We have previously shown in Holothuria leucospilota the maturation-inducing role of the neurohormone relaxin-like gonad-stimulating peptide (RGP). Here, we report the induction of oocyte maturation and spawning in another holothuroid, the white teatfish, H. fuscogilva, with a species-specific recombinant RGP produced in the yeast expression system. The recombinant H. fuscogilva RGP (rwt_RGP) was bioactive in vitro, as demonstrated in germinal vesicle breakdown (GVBD) assays, and in vivo, stimulating spawning in H. fuscogilva at three doses tested (50, 100 and 150 μg/kg). The spawned gametes were viable, and here we report their development until the early juvenile stage. The rwt_RGP was also..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This research was funded by Aus4Innovation CSIRO Hanoi (grant number A4I120) . Further funding was provided by the University of the Sunshine Coast (USC) and Research Institute for Aquaculture 3 (RIA3) . We thank Tasmanian Seafoods for generously providing white teatfish tissue samples and testing of recombinant and synthetic peptides, Mr. Adam Hillberg for assistance with timeframe photography and Dr. Lachlan P. Dennis for assistance with in-vitro assays at USC and Drs Wayne Knibb and Kelli Anderson for statistical analysis. RADB is an NHMRC Senior Research Fellow at the Florey Institute of Neuroscience and Mental Health was supported by the State Government of Victoria Operational Infrastructure Support Program. We are very grateful to Oscar Liu, Florey Institute of Neuroscience and Mental Health, for assistance with peptide synthesis.