Journal article

Spawning induction of the high-value white teatfish sea cucumber, Holothuria fuscogilva, using recombinant relaxin-like gonad stimulating peptide (RGP)

J Nocillado, NDQ Duy, HD Chieu, L Turner, RAD Bathgate, T Wang, MA Hossain, NV Hung, NH Ninh, SF Cummins, A Elizur

Aquaculture | Elsevier BV | Published : 2022

Abstract

We have previously shown in Holothuria leucospilota the maturation-inducing role of the neurohormone relaxin-like gonad-stimulating peptide (RGP). Here, we report the induction of oocyte maturation and spawning in another holothuroid, the white teatfish, H. fuscogilva, with a species-specific recombinant RGP produced in the yeast expression system. The recombinant H. fuscogilva RGP (rwt_RGP) was bioactive in vitro, as demonstrated in germinal vesicle breakdown (GVBD) assays, and in vivo, stimulating spawning in H. fuscogilva at three doses tested (50, 100 and 150 μg/kg). The spawned gametes were viable, and here we report their development until the early juvenile stage. The rwt_RGP was also..

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