Journal article

Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions

Andrew H Buultjens, Koen Vandelannoote, Liam K Sharkey, Benjamin P Howden, Ian R Monk, Jean YH Lee, Timothy P Stinear

ACS BIOMATERIALS SCIENCE & ENGINEERING | AMER CHEMICAL SOC | Published : 2021

Abstract

The ability to detect SARS-CoV-2 is critical to implementing evidence-based strategies to address the COVID-19 global pandemic. Expanding SARS-CoV-2 diagnostic ability beyond well-equipped laboratories widens the opportunity for surveillance and control efforts. However, such advances are predicated on the availability of rapid, scalable, accessible, yet high-performance diagnostic platforms. Methods to detect viral RNA using reverse transcription loop-mediated isothermal amplification (RT-LAMP) show promise as rapid and field-deployable tests; however, the per-unit costs of the required diagnostic hardware can be a barrier for scaled deployment. Here, we describe a diagnostic hardware confi..

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